Main Line of Research

Lines of Research

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The research activities have resulted in scientific papers published in peer-reviewed journals (Am J Pathol, J Pathol, Lab Invest, Diagn Mol Pathol, Am J Surg Pathol, Hum Pathol, Am J Clin Pathol, J Clin Endocrinol Metab, J Endocrinol, Gastroenterology, Worl J Gastroenterol, Br J Dermatol, Arch Dermatol), mainly comprising molecular aspects of neoplasms (clonality and progression) in three main areas of interest endocrine pathology, dermatopathology and transitional cell carcinomas of the bladder, and abstracts accepted in National and International Pathology Meetings. It has also resulted in invited lectures in Pathology Meetings. This research has been financed by Departamental funding, Grant for PhD studies provided by the Egyptian Government, joint grant applications with Endocrinology Depertment at Barts and The London School of Medicine and Dentistry, and European Funds for Regional Development as part of a joint proposal to develop a Molecular and Cellular Pathology laboratory at the Research Center for Biological and Health Sciences (University of Malaga School of Medicine).


Publications comprise 3 monographs (Ph.D. Doctoral Thesis, Teaching Portfolio and Programme on Pathology for undergraduate and postgraduate students, and the edition of the International Symposium on Nervous System Tumors), 5 book chapters, and 97 articles in peer-reviewed medical journals (15 review articles and 83 original papers), for a total of 106 scientific publications. The articles cover technical and methodological aspects, diagnosis, and prognosis in pathology, especially oncologic pathology. Those aspects are mainly concentrated on in situ techniques, molecular techniques, and conventional histopathology. Links to relevant papers are available from PubMed (National Library of Medicine).

This research has been especially focused on kinetic and molecular aspects for the topographic evolution of solid neoplasms. These publications would represent a generic research project I have develop during the last decade:

Topographic Tumor Heterogeneity: Biological Bases and Applications

1 - Clonal origin and expansions in tumors - (Diaz-Cano 1996; Alman, Pajerski et al. 1997; Diaz-Cano and Brady 1997; Diaz-Cano and Wolfe 1997; Diaz-Cano 1998; Diaz-Cano 2000; Diaz-Cano, de Miguel et al. 2000; Diaz-Cano, Blanes et al. 2001; Pozo-Garcia and Diaz-Cano 2003; Diaz-Cano 2008)

2 - Cell kinetics in tumors - (Diaz-Cano, Gonzalez-Campora et al. 1993; Gonzalez-Campora, Diaz Cano et al. 1993; Salomon and Diaz-Cano 1995; Diaz-Cano, Garcia-Moliner et al. 1997; Diaz-Cano, de Miguel et al. 2000; Koch, de Miguel et al. 2000; Diaz-Cano, de Miguel et al. 2001; Vax, Bibi et al. 2003; Arif, Patel et al. 2007; Blanes and Diaz-Cano 2007; Pozo, Husein et al. 2008)

3 - Tumor cell heterogeneity

a - Mechanisms, instability and segregation of tumor cells by cell kinetics - (Alman, Li et al. 1997; Diaz-Cano, de Miguel et al. 2000; Smits, Ruiz et al. 2000; Diaz-Cano, de Miguel et al. 2001; Blanes and Diaz-Cano 2006; Blanes and Diaz-Cano 2006)

4 - Topographic tumor compartments – Genetic and kinetic profiles - (Diaz-Cano, Blanes et al. 2000; Baithun, Naase et al. 2001; Diaz-Cano, de Miguel et al. 2001; Blanes, Rubio et al. 2002; Jimenez, Blanes et al. 2003; Blanes and Diaz-Cano 2006; Blanes, Sanchez-Carrillo et al. 2006; Diaz-Cano 2006; Diaz-Cano 2007; Pozo, Sanchez-Carrillo et al. 2007; Rubio, Blanes et al. 2007; Ellis, Cano et al. 2008; Blanes, Rubio et al. 2009)

5 - Pathologic models

a - Luminal organs: Bladder, colon, cutaneous (melanocytic, adnexal)

I - Intraepithelial, superficial and deep compartments - (Diaz-Cano, Blanes et al. 2000; Baithun, Naase et al. 2001; Blanes, Rubio et al. 2002; Blanes and Diaz-Cano 2006; Rubio, Blanes et al. 2007) (Ellis, Cano et al. 2008; Blanes, Rubio et al. 2009)

b - Solid organs: Thyroid, adrenal cortex, adrenal medulla

I - Peripheral and internal compartments - (Diaz-Cano, Gonzalez-Campora et al. 1993; Diaz-Cano, de Miguel et al. 2000; Diaz-Cano, de Miguel et al. 2000; Diaz-Cano, de Miguel et al. 2001; Blanes and Diaz-Cano 2006; Blanes, Sanchez-Carrillo et al. 2006; Diaz-Cano 2006; Arif, Patel et al. 2007; Diaz-Cano 2007)

c - Inherited cancer syndromes: RET point mutation model

I - C-cell hyperplasia/medullary thyroid carcinoma - (Diaz-Cano, de Miguel et al. 2001; Mears and Diaz-Cano 2003)

II - Adrenal medullary hyperplasia/pheochromocytoma - (Diaz-Cano 1998; Diaz-Cano, de Miguel et al. 2000; Blanes, Sanchez-Carrillo et al. 2006)


These research articles can be downloaded in generic groups from the links provided: 

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References

Blanes, A., J. Rubio, J. J. Sanchez-Carrillo and S. J. Diaz-Cano (2009). "Coexistent intraurothelial carcinoma and muscle-invasive urothelial carcinoma of the bladder: clonality and somatic down-regulation of DNA mismatch repair." Hum Pathol 40(7): 988-997. PubMed Abstract

Diaz-Cano, S. J. (2008). "Motesanib Diphosphate in Progressive Differentiated Thyroid Cancer." New England Journal of Medicine 359(25): 2727-2727. PubMed Abstract

Ellis, P. E., S. D. Cano, M. Fear, D. P. Kelsell, L. Ghali, J. C. Crow, C. W. Perrett and A. B. MacLean (2008). "Reduced E-cadherin expression correlates with disease progression in Paget's disease of the vulva but not Paget's disease of the breast." Mod Pathol 21(10): 1192-1199. PubMed Abstract

Pozo, L., E. Husein, A. Blanes and S. J. Diaz-Cano (2008). "The correlation of regression with the grade of dysplasia (atypia) in melanocytic naevi." Histopathology 52(3): 387-389. PubMed Abstract

Arif, S., J. Patel, A. Blanes and S. J. Diaz-Cano (2007). "Cytoarchitectural and kinetic features in the histological evaluation of follicular thyroid neoplasms." Histopathology 50(6): 750-763. PubMed Abstract 

Blanes, A. and S. J. Diaz-Cano (2007). "Histologic criteria for adrenocortical proliferative lesions: value of mitotic figure variability." Am J Clin Pathol 127(3): 398-408. PubMed Abstract

Diaz-Cano, S. J. (2007). "Kinetic topographical heterogeneity in follicular thyroid neoplasms and growth patterns." Histopathology 51(3): 416-418. PubMed Abstract

Pozo, L., J. Sanchez-Carrillo, A. Martinez, A. Blanes and S. Diaz-Cano (2007). "Differential kinetic features by tumour topography in cutaneous small-cell neuroendocrine (Merkel cell) carcinomas." J Eur Acad Dermatol Venereol 21(9): 1220-1228. PubMed Abstract

Rubio, J., A. Blanes, J. J. Sanchez-Carrillo and S. J. Diaz-Cano (2007). "Microsatellite abnormalities and somatic down-regulation of mismatch repair characterize nodular-trabecular muscle-invasive urothelial carcinoma of the bladder." Histopathology 51(4): 458-467. PubMed Abstract

Blanes, A. and S. J. Diaz-Cano (2006). "Complementary analysis of microsatellite tumor profile and mismatch repair defects in colorectal carcinomas." World J Gastroenterol 12(37): 5932-5940. PubMed Abstract 

Blanes, A. and S. J. Diaz-Cano (2006). "DNA and kinetic heterogeneity during the clonal evolution of adrenocortical proliferative lesions." Hum Pathol 37(10): 1295-1303. PubMed Abstract 

Blanes, A., J. J. Sanchez-Carrillo and S. J. Diaz-Cano (2006). "Topographic molecular profile of pheochromocytomas: role of somatic down-regulation of mismatch repair." J Clin Endocrinol Metab 91(3): 1150-1158. PubMed Abstract

Diaz-Cano, S. J. (2006). "Molecular mechanisms in melanoma." N Engl J Med 355(13): 1395; author reply 1396. PubMed Abstract

Diaz-Cano, S. J. (2006). "Re: Pomerance et al. High-level expression, activation, and subcellular localization of p38-MAP kinase in thyroid neoplasms. J Pathol 2006; 209: 298-306." J Pathol 210(1): 133-134. PubMed Abstract

Jimenez, J. J., A. Blanes and S. J. Diaz-Cano (2003). "Microsatellite instability in colon cancer." N Engl J Med 349(18): 1774-1776; author reply 1774-1776. PubMed Abstract

Mears, L. and S. J. Diaz-Cano (2003). "Difference between familial and sporadic medullary thyroid carcinomas." American Journal of Surgical Pathology 27(2): 266-267. PubMed Abstract

Pozo-Garcia, L. and S. J. Diaz-Cano (2003). "Clonal origin and expansions in neoplasms: biologic and technical aspects must be considered together." Am J Pathol 162(1): 353-354; author reply 354-355. PubMed Abstract

Vax, V. V., R. Bibi, S. Diaz-Cano, M. Gueorguiev, B. Kola, N. Borboli, B. Bressac-De Paillerets, G. J. Walker, Dedov, II, A. B. Grossman and M. Korbonits (2003). "Activating point mutations in cyclin-dependent kinase 4 are not seen in sporadic pituitary adenomas, insulinomas or Leydig cell tumours." J Endocrinol 178(2): 301-310. PubMed Abstract

Blanes, A., J. Rubio, A. Martinez, H. J. Wolfe and S. J. Diaz-Cano (2002). "Kinetic profiles by topographic compartments in muscle-invasive transitional cell carcinomas of the bladder: role of TP53 and NF1 genes." Am J Clin Pathol 118(1): 93-100. PubMed Abstract

Baithun, S. I., M. Naase, A. Blanes and S. J. Diaz-Cano (2001). "Molecular and kinetic features of bladder transitional cell carcinomas. Biological and clinical implications." Virchows Arch 438(3): 289-297. PubMed Abstract

Diaz-Cano, S. J., A. Blanes and H. J. Wolfe (2001). "PCR techniques for clonality assays." Diagn Mol Pathol 10(1): 24-33. PubMed Abstract

Diaz-Cano, S. J., M. de Miguel, A. Blanes, H. Galera and H. J. Wolfe (2001). "Contribution of the microvessel network to the clonal and kinetic profiles of adrenal cortical proliferative lesions." Hum Pathol 32(11): 1232-1239. PubMed Abstract

Diaz-Cano, S. J., M. de Miguel, A. Blanes, R. Tashjian and H. J. Wolfe (2001). "Germline RET 634 mutation positive MEN 2A-related C-cell hyperplasias have genetic features consistent with intraepithelial neoplasia." J Clin Endocrinol Metab 86(8): 3948-3957. PubMed Abstract

Diaz-Cano, S. J. (2000). "Designing a molecular analysis of clonality in tumours." J Pathol 191: 343-344. PubMed Abstract

Diaz-Cano, S. J., A. Blanes, J. Rubio, A. Matilla and H. J. Wolfe (2000). "Molecular evolution and intratumor heterogeneity by topographic compartments in muscle-invasive transitional cell carcinoma of the urinary bladder." Lab Invest 80(3): 279-289. PubMed Abstract

Diaz-Cano, S. J., M. de Miguel, A. Blanes, R. Tashjian, H. Galera and H. J. Wolfe (2000). "Clonal patterns in phaechromocytomas and MEN-2A adrenal medullary hyperplasias: histologic and kinetic correlates." J Pathol 192(2): 221-228. PubMed Abstract

Diaz-Cano, S. J., M. de Miguel, A. Blanes, R. Tashjian, H. Galera and H. J. Wolfe (2000). "Clonality as expression of distinctive cell kinetics patterns in nodular hyperplasias and adenomas of the adrenal cortex." Am J Pathol 156(1): 311-319. PubMed Abstract

Koch, M., M. de Miguel, H. Höfler and S. J. Diaz-Cano (2000). "Kinetic profiles of intraepithelial and invasive prostatic neoplasias: the key role of down-regulated apoptosis in tumor progression." Virchows Arch 436(5): 413-420. PubMed Abstract

Smits, R., P. Ruiz, S. Diaz-Cano, A. Luz, S. Jagmohan-Changur, C. Breukel, C. Birchmeier, W. Birchmeier and R. Fodde (2000). "E-cadherin and adenomatous polyposis coli mutations are synergistic in intestinal tumor initiation in mice." Gastroenterology 119(4): 1045-1053. PubMed Abstract

Diaz-Cano, S. J. (1998). "Clonality Studies in the Analysis of Adrenal Medullary Proliferations: Application Principles and Limitations." Endocr Pathol 9(1): 301-316. PubMed Abstract

Alman, B. A., C. Li, M. E. Pajerski, S. Diaz-Cano and H. J. Wolfe (1997). "Increased beta-catenin protein and somatic APC mutations in sporadic aggressive fibromatoses (desmoid tumors)." Am J Pathol 151(2): 329-334. PubMed Abstract 

Alman, B. A., M. E. Pajerski, S. Diaz-Cano, K. Corboy and H. J. Wolfe (1997). "Aggressive fibromatosis (desmoid tumor) is a monoclonal disorder." Diagn Mol Pathol 6(2): 98-101. PubMed Abstract 

Diaz-Cano, S. J. and S. P. Brady (1997). "DNA extraction from formalin-fixed, paraffin-embedded tissues: protein digestion as a limiting step for retrieval of high-quality DNA." Diagn Mol Pathol 6(6): 342-346. PubMed Abstract 

Diaz-Cano, S. J., M. Garcia-Moliner, W. Carney and H. J. Wolfe (1997). "Bcl-2 expression and DNA fragmentation in breast carcinoma, pathologic and steroid hormone receptors correlates." Diagn Mol Pathol 6(4): 199-208. PubMed Abstract 

Diaz-Cano, S. J. and H. J. Wolfe (1997). "Clonality in Kaposi's sarcoma [letter; comment]." N Engl J Med 337(8): 571-572. PubMed Abstract

Diaz-Cano, S. (1996). "PCR-based alternative for diagnosis of immunoglobulin heavy chain gene rearrangement: principles, practice, and polemics." Diagn Mol Pathol 5(1): 3-9. PubMed Abstract 

Salomon, R. N. and S. Diaz-Cano (1995). "Introduction to apoptosis." Diagn Mol Pathol 4(4): 235-238. PubMed Abstract 

Diaz-Cano, S., R. Gonzalez-Campora, J. J. Rios-Martin, E. Lerma-Puertas, M. Jorda-Heras, F. Vazquez-Ramirez, M. Bibbo and H. Galera-Davidson (1993). "Nuclear DNA patterns in adrenal cortex proliferative lesions." Virchows Arch A Pathol Anat Histopathol 423(5): 323-328. PubMed Abstract 

Gonzalez-Campora, R., S. Diaz Cano, E. Lerma-Puertas, J. J. Rios Martin, M. Salguero Villadiego, J. L. Villar Rodriguez, M. Bibbo and H. G. Davidson (1993). "Paragangliomas. Static cytometric studies of nuclear DNA patterns." Cancer 71(3): 820-824. PubMed Abstract 

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